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Anti-Fibrillarin antibody - Nucleolar Marker
Anti-Fibrillarin antibody - Nucleolar Marker from Abcam
Be the first to write a review!
Citations:
(177)
Supplier Page
Supplier Page from
Abcam for
Anti-Fibrillarin antibody - Nucleolar Marker
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
Anti-Fibrillarin antibody - Nucleolar Marker
Company
Abcam
Price
Pricing Info
Supplier Page
View Supplier Product Page
Catalog Number
ab5821
Antibody Type
Primary
Quantity
100 UG
Isotype
IgG
Clonality
Polyclonal
Applications
Immunocytochemistry/ Immunofluorescence, Western blot
Host
Rabbit
Conjugate/Tag
Unconjugated
Concentration
0.5 mg/ml
Research Area
Epigenetics
Gene Name
fbl
Reactivity
Drosophila melanogaster, Human, Mouse
Target
Fibrillarin
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (177)
(1)
Chromosome-specific maturation of the epigenome in the Drosophila male germline
eLife
November 30, 2023
James T Anderson, Steven Henikoff, Kami Ahmad
Cell Signalling Technology C36B11 anti-H3-K27-trimethyl,
Abcam
ab109463
anti-H4-K16-acetyl, and
Abcam
ab5821
anti-fibrillarin. Imaging whole testes Testes from 1-day-old adult males were dissected and fixed
(2)
NAD+ regulates nucleotide metabolism and genomic DNA replication
Nature Cell Biology
November 13, 2023
Sebastian Howen Nesgaard Munk, Joanna Maria Merchut-Maya, Alba Adelantado Rubio, Arnaldur Hall, George Pappas, Giacomo Milletti, MyungHee Lee, Lea Giørtz Johnsen, Per Guldberg, Jiri Bartek,
et al.
detection of BrUTP incorporation into nascent transcripts together with an anti-Fibrillarin antibody (
Abcam
ab5821
,
1:500
). Images were acquired on a LSM800
confocal
microscope with a Plan-Apochromat 63x/1.4 numerical
(3)
Affinity hierarchies and amphiphilic proteins underlie the co-assembly of nucleolar and heterochromatin condensates
Research Square
November 9, 2023
Srivarsha Rajshekar, Srivarsha Adame-Arana, Gaurav Adame-Arana, Gaurav Bajpai, Serafin Colmenares, Serafin Colmenares, Samuel Safran
Reagent or Resource,Source,Identifier Antibodies,, Rabbit anti-Fibrillarin,
Abcam
,
ab5821
Mouse
anti-H3K9me2,
Abcam
,
ab8898
Mouse
anti-Modulo,"Gift from Mellone Lab (gift of Jacques Pradel,
(4)
Affinity hierarchies and amphiphilic proteins underlie the co-assembly of nucleolar and heterochromatin condensates
bioRxiv
September 30, 2023
Srivarsha Rajshekar, Omar Adame-Arana, Gaurav Bajpai, Kyle Lin, Serafin Colmenares, Samuel Safran, Gary H Karpen
Reagent or Resource,Source,Identifier Antibodies,, Rabbit anti-Fibrillarin,
Abcam
,
ab5821
Mouse
anti-H3K9me2,
Abcam
,
ab8898
Mouse
anti-Modulo,"Gift from Mellone Lab (gift of Jacques Pradel,
(5)
Human nucleolar protein SURF6/RRP14 participates in early steps of pre-rRNA processing
PLOS ONE
July 14, 2023
Anastasiia Moraleva, Alexander Deryabin, Maria Kordyukova, Mikhail Polzikov, Kseniya Shishova, Kira Dobrochaeva, Yury Rubtsov, Maria Rubtsova, Olga Dontsova, Olga Zatsepina
antibodies to
SURF6
[21] (diluted
1:100
) and rabbit antibodies to Fibrillarin (diluted
1:200
;
Abcam
,
ab5821
) for 1h at room temperature. Then washed for 3x5 min in PBS and stained with a mixture of antibodies
(6)
Centromere proteins are asymmetrically distributed between newly divided germline stem and daughter cells and maintain a balanced niche in Drosophila males
Molecular Biology of the Cell
May 1, 2023
Antje M. Kochendoerfer, Rachel S. Keegan, Elaine M. Dunleavy
1:700
),
mouse
anti-1B1 (
Hts
; DSHB;
1:100
),
mouse
anti-BAM (DSHB;
1:50
), mouse anti-FIBRILLARIN (
Abcam
;
ab5821
;
1:500
), mouse anti-HA (
ThermoFisher
;
26183
;
1:500
),
mouse
anti-gamma-tubulin (
Abcam
;
ab44928
;
1:500
),
(7)
DDX47, MeCP2, and other functionally heterogeneous factors protect cells from harmful R loops
Cell reports
March 28, 2023
Marchena-Cruz E, Camino LP, Bhandari J, Silva S, Marqueta-Gracia JJ, Amdeen SA, Guillén-Mendoza C, García-Rubio ML, Calderón-Montaño JM, Xue X,
et al
.
Anti-FLAG,
Abcam
,Cat#
ab1257
;RRID: AB_299216 Polyclonal antibody Rabbit anti-Fibrillarin,
Abcam
,Cat#
ab5821
;RRID:AB_2105785
Monoclonal antibody
anti-Histidine
,
Sigma
,Cat# GE27-4710-01 Polyclonal antibody Rabbit
(8)
A Dynamic rRNA Ribomethylome Drives Stemness in Acute Myeloid Leukemia
Cancer discovery
February 6, 2023
Zhou F, Aroua N, Liu Y, Rohde C, Cheng J, Wirth AK, Fijalkowska D, Göllner S, Lotze M, Yun H,
et al
.
in PBS containing 0.5% (v/v)
Tween-20
with 5% milk powder. Primary antibodies against anti-FBL (cat.
ab5821
,
Abcam
), anti-V5 tag (cat.
ab9116
,
Abcam
), anti–β-Actin (cat.
ab6276
,
Abcam
), anti-
PRPF39
(cat.
GTX104949
,
(9)
Expression of RNA polymerase I catalytic core is influenced by RPA12
PloS one
January 1, 2023
Ford BL, Wei T, Liu H, Scull CE, Najmi SM, Pitts S, Fan W, Schneider DA, Laiho M.
monoclonal D10
1:100
;
sc-393406
Santa Cruz Biotechnology
) and fibrillarin (rabbit polyclonal
1:500
;
ab5821
Abcam
). Secondary antibodies used were Alexa 488 and Alexa 594-conjugated anti-
mouse
and anti-rabbit
(10)
Nucleophagy delays aging and preserves germline immortality
Nat Aging
January 1, 2023
Papandreou ME, Konstantinidis G, Tavernarakis N.
anti-p62/SQSTM1 (catalog no.
GP62-C
;
Progen
); rabbit anti-Syne-1 or rabbit anti-fibrillarin (catalog no.
ab5821
;
Abcam
). Coverslips were then washed with PBS, followed by secondary antibody
staining
for 1 h at room
(11)
MALAT1-dependent hsa_circ_0076611 regulates translation rate in triple-negative breast cancer
Communications Biology
December 1, 2022
Chiara Turco, Gabriella Esposito, Alessia Iaiza, Frauke Goeman, Anna Benedetti, Enzo Gallo, Theodora Daralioti, Letizia Perracchio, Andrea Sacconi, Patrizia Pasanisi,
et al.
(
Cell Signaling
: #
2469
), anti-SRSF1 (
Santa Cruz
:
SF2
/ASF96
sc-33652
) and anti-Fibrillarin (
Abcam
: FBL
ab5821
) were used. ChIRP
assay
of circ_0076611, RNAseq and bioinformatic analysis ChIRP was performed as
(12)
Low level of Fibrillarin, a ribosome biogenesis factor, is a new independent marker of poor outcome in breast cancer
BMC Cancer
December 1, 2022
Flora Nguyen Van Long et al.
immunohistochemical staining
FBL
immunohistochemistry (IHC
) was performed using two different lots of FBL antibody for CLB-1 (
ab5821
lot GR1979-001,
Abcam
) and IGR-1 series (
ab5821
lot GR253838-1, Abcam). FBL
staining
corresponds to
(13)
HITS-CLIP analysis of human ALKBH8 reveals interactions with fully processed substrate tRNAs and with specific noncoding RNAs
RNA
December 1, 2022
Ivana Cavallin, Marek Bartosovic, Tomas Skalicky, Praveenkumar Rengaraj, Martin Demko, Martina Christina Schmidt-Dengler, Aleksej Drino, Mark Helm, Stepanka Vanacova
Sigma Aldrich
F3165
), anti-tubulin (
1:10,000
,
Sigma Aldrich
T6074
), anti-fibrillarin (
1:1000
,
Abcam
ab5821
), anti-
ALKBH8
(
1:500
,
Abcam
ab113512
). Washes and
incubation
of HRP-conjugated secondary antibodies
(14)
A Spontaneous Inversion of the X Chromosome Heterochromatin Provides a Tool for Studying the Structure and Activity of the Nucleolus in Drosophila melanogaster
Cells
December 1, 2022
Tatyana D. Kolesnikova, Mikhail S. Klenov, Alina R. Nokhova, Sergey A. Lavrov, Galina V. Pokholkova, Veit Schubert, Svetlana V. Maltseva, Kevin R. Cook, Michael J. Dixon, Igor F. Zhimulev
were performed according to the protocol described in [38]. Rabbit polyclonal anti-Fibrillarin (
Abcam
,
ab5821
,
1:5000
) or
guinea pig
anti-Udd (
1:300
, [46], kindly provided by M. Buszczak) primary antibodies and
(15)
AATF /Cheâ€1 localizes to paraspeckles and suppresses Râ€loops accumulation and interferon activation in Multiple Myeloma
The EMBO Journal
November 17, 2022
Tiziana Bruno, Giacomo Corleone, Valeria Catena, Clelia Cortile, Francesca De Nicola, Francesca Fabretti, Svitlana Gumenyuk, Francesco Pisani, Andrea Mengarelli, Claudio Passananti,
et al.
anti‐αTubulin (DM1A) (WB
1:500
),Calbiochem,Cat# CP06 Rabbit Polyclonal anti‐Fibrillarin (IF
1:100
),
Abcam
,Cat#
ab5821
Alexa Fluor 594‐anti‐Rabbit (IF
1:500
),
ThermoFisher
,Cat#
A11037
Alexa Fluor 594‐anti‐Mouse (IF
1:500
),
ThermoFisher
,Cat#
(16)
Control of ribosomal RNA synthesis by hematopoietic transcription factors
Molecular cell
October 20, 2022
Antony C, George SS, Blum J, Somers P, Thorsheim CL, Wu-Corts DJ, Ai Y, Gao L, Lv K, Tremblay MG,
et al
.
manufacturer protocol. Precleared cell extract was incubated with 5 µg of anti-Fibrillarin (
Abcam
, #
ab5821
), or anti-FLAG-M2 (Sigma, #
F1804
) antibodies, or Rabbit Polyclonal IgG (
Invitrogen
, #
02–6102
), or
Mouse
(17)
Epithelial cell size dysregulation in human lung adenocarcinoma
PLOS ONE
October 6, 2022
Clifford W. Sandlin, Song Gu, Jun Xu, Charuhas Deshpande, Michael D. Feldman, Matthew C. Good
A+C),Rabbit,Monoclonal/-,
Abcam
,
ab108595
,1° IF /
1:200
Proprietary synthetic Fibrillarin peptide (Fibrillarin),Rabbit,Polyclonal/-,
Abcam
,
ab5821
,1° IF /
1:200
Human
SON/DBP5 1463–1598 (SON),Rabbit,Polyclonal/-,
Abcam
,
ab121759
,1° IF /
1:200
"Keratin,
(18)
Adeno-associated virus type 2 (AAV2) uncoating is a stepwise process and is linked to structural reorganization of the nucleolus
PLOS Pathogens
July 11, 2022
Sereina O. Sutter, Anouk Lkharrazi, Elisabeth M. Schraner, Kevin Michaelsen, Anita Felicitas Meier, Jennifer Marx, Bernd Vogt, Hildegard Büning, Cornel Fraefel
1:10
),
anti-AAV2
Rep (
Fitzgerald Industries
,
10R-A111A
, dilution for IF
1:10
), anti-fibrillarin (
Abcam
ab5821
; dilution for IF
1:200
; dilution
for Western blotting
[WB]
1:650
), anti-cyclin A (
Santa Cruz
sc-751
,
(19)
Vulnerability of HIF1α and HIF2α to damage by proteotoxic stressors
Toxicology and Applied Pharmacology
June 15, 2022
Lauren M. Meyers, Casey Krawic, Michal W. Luczak, Anatoly Zhitkovich
ImmunoBlot
polyvinylidene difluoride
membranes
(
1620177
,
Bio-Rad
). Primary antibodies: fibrillarin (
ab5821
) and phospho-S326-HSF1 (
ab76076
) from
Abcam
; HIF1α (
610958
) from
BD Biosciences
; HA-tag (3724), HIF1α
(20)
Promyelocytic leukemia nuclear body-like structures can assemble in mouse oocytes
Biology Open
June 15, 2022
Osamu Udagawa, Ayaka Kato-Udagawa, Seishiro Hirano
telangiectasia
(CREST)
human
autoantibody (HCT-0100: Immuno Vision, Springdale, USA), anti-fibrillarin (
ab5821
), anti-SUMO-conjugating enzyme (Ubc9), anti-proteasome 20S alpha 1+2+3+5+6+7 (20S) (
Abcam
, Cambridge,
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Anti-Fibrillarin antibody - Nucleolar Marker from Abcam
Supplier Page
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Pricing Info
Product Specs
Company Info
Citations
Reviews
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